Biological Labs
Room 2052
16 Divinity Ave.
Cambridge, MA 02138
Director of Imaging
Douglas Richardson, PhD
Biolabs 2052B

Zeiss Specialist
Casey Kraft, PhD
Biolabs 2052A

Support Specialist
David Smith
Biolabs 2052

Tu & W, 9 am - 5 pm

Registration, Fees,
Billing, and SPINAL
Sarah Elwell
NWL B227.45


Faculty Director
Prof. Jeff Lichtman
Harvard Center for Biological Imaging
  • Lightsheet microscopy at the HCBI was recently featured in BioOptics World. Click here to read the article!
  • Get to know the newest Evergreen microscopes: Axio Scan.Z1 high-throughput slide scanner, Lightsheet Z.1 for rapid 3D volume imaging, and the LSM 780 upright NLO for multi-photon deep tissue imaging. Email Doug Richardson to set up training on any one of these systems.
  • Confused about mounting media? Click here for the HCBI's recommendations!
  • Do you want to image in MILLImeters and not MICROmeters? Check out these 4 papers, then come talk to us! SCALE reagent, CLARITY method, SeeDB reagent, TDE reagent.
  • Download the list of required consumables for Lightsheet experiments here.
  • View a video of Lightsheet sample prep here. Can you spot the embryo imaged by Katherine Rogers at the HCBI?
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Stephen Sontoro (Dulac lab)

A 10 micrometer cryosection of the mouse olfactory epithelium. Sensory neuron nuclei are stained with antibodies that recognize an olfactory-specific histone H2B variant (red), which participates in regulating the rate of neuronal turnover, and ubiquitinated H2B (green), a modification that participates in the regulation of transcription. All cell nuclei are labeled with DAPI (blue).

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Stephen Von Stetina (Mango lab)

A 2-fold C. elegans embryo showing internal epithelia marked with the adherens junction protein Discs Large (green), localized to the apical/lateral surface of pharyngeal (between arrows) and intestinal (between arrowheads) epithelia. UNC-70/beta-spectrin (red) labels the cortex of all cell membranes. Anterior to the left.

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Albert Pan (Schier lab)

This photo shows the side view of the head region of a 3 day old larval transgenic zebrafish. This fish has green fluorescent protein (GFP) in neurons in the brain, eyes, and sensory neurons (adjacent to the eye). For better visualization, this larvae was stained to show GFP (green) and other cells in the nervous system (red and blue)

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Ryan Draft (Lichtman lab)

Multicolor motor neuron axons synapsing onto the mouse Omohyoid muscle.

Axons were labeled using the Brainbow method (Livet et al, 2007) and imaged at high spatial and spectral resolution with the Omlympus FV 1000 confocal microscope. The many resulting fields of view were stitched together into a single montage and displayed as a maximum intensity projection.

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Robert Kao (McMahon lab)

Image is taken from a fluorescent mouse embryonic kidney showing cells from the distal end of the nephron (in green), and the red part is from the collecting duct. The C shaped green cells are another cell population called the condensed mesenchyme.

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Geoff Lau (Murthy lab)

Excitatory and inhibitory neurons from mouse brain forming synaptic connections with each other. Green: GFP fluorescence present exclusively in inhibitory neurons; blue: cellular nuclei; red: synapses

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Jose Rivera-Feliciano (Melton lab)

Indirect immunofluorescence staining depicting the micro-vascular network of the Islets of Langerhans.

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Peng Huang (Schier lab)

The picture shows cilia in a 1-somite stage zebrafish embryo. Cilia are labeled by rSmoM2-EGFP (green), and the cell membrane is labeled with membrane-targeted mRFP

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Sam Kunes (Kunes lab)

A view of the developing optic ganglia of a fruitfly brain, showing the regular array of retinal axons eye (green stain) as they emerge from the optic nerve and spread out among the differentiating neurons (red stain) and glia (blue stain) of the optic ganglia. Neurons, glia and precursor cells destined for elimination by apoptosis are all arranged in layers perpendicular to the retinal axons.