Colony PCR

Preparation

  • Streak out yeast and grow until small colonies form.

Procedure

  1. Place 5 ul of water into a PCR tube.
  2. Stick a pipette tip into the colony to pick up a small number of cells. Smash the pipette tip into the cells.
  3. Freeze the PCR tube for 10-15 min.
  4. Microwave the PCR tube for 1 min on high power.
  5. Freeze the PCR tube for 10-15 min.
  6. Prepare 45 ul of the PCR mix.
  7. Add the PCR mix to the tube, mix, spin down, and PCR as normal.

The Murray Lab, Harvard University, Dept. of Molecular and Cellular Biology, 52 Oxford St Room 469, Cambridge, MA 02138