Temporal dynamics of flagellar promoters
We study the temporal dynamics within the flagellum assembly cascade in E. coli at the single-cell level. The assembly of the flagellum’s basal body, hook, and chemotaxis system is governed by an intricate but closed system of feedback loops. The cell uses the incomplete flagellum as a pump to extricate the anti-sigma factor flgM, which blocks completion of the flagellum, at the appropriate time. Population-level studies have revealed potential ordering schemes, but single-cell experiments monitoring multiple promoters should reveal the relevant time scales and role of molecular memory within the system.
This project is the work of graduate student Mark Kim.